![]() ![]() ![]() The women in The Beach House could be seen as existing on the same plane of female experience but at different points in their lives: daughters, mistresses, wives, single parents, widows. I don’t ever stop to analyze whether something makes sense-when I’m writing characters, and particularly someone as vivid as Nan, they become their own people, and I allow them, as pretentious as it may sound, to dictate their own behavior. I think my characters all share similar sensibilities, and perhaps the only real difference with Nan is that age has given her a wisdom and an acceptance that some of the younger characters might not have. Is it possible to read Nan as an older version of any of the younger heroines you’ve written about in earlier books? Are there any different considerations necessary when writing about an older character? Throughout your work, you’ve created a number of memorable heroines. I was fascinated by her, and certainly held that image of her while I was creating Nan. Part of our courtship involved long walks by the beach at midnight, and there was a fabulously glamorous woman of a certain age whom we used to see riding her bike, cigarette in hand, at one or two in the morning. When I first moved into my tiny cottage by the beach the summer of my divorce, I found myself falling slowly in love with my landlord (we now live together, in a bigger house a couple of blocks away). Where did you find your inspiration for this particular book? Is Nan based on anyone in your own life?
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![]() 12 device simulations eliminate the need to export audio to check playback. Buy MixChecker Pro by Audified - 145.00 - Mastering. Compatibility with host presets and stored plugin state made with older versions is not guaranteed. No impulse responses anymore, there is a new phase-linear, low latency engine behind the simulation. ![]() MixChecker Pro simulates more than 60 devices and you may create custom sets from them with automatic advancing. Device compensation makes sure your studio setup accurately reproduces sound. Former simulations replaced by high-quality simulations from the Pro version the linear part only. Audified has announced the release of the new MixChecker Pro. AUDIFIED MixChecker Pro - Monitor Simulation Plug-In (Download) Simulate Professional Reference Monitors Simulate Headphones, Earphones Simulate Smartphone. Audified MixChecker Plug-in Features: Checks your mix against common consumer devices to bring the best quality to your listeners. It improves on its sibling with many more convolution-based speaker sims, nonlinear. A more advanced version of its MixChecker plugin effect, Audified’s MixChecker Pro (VST/AU/AAX) simulates a wide range of playback speaker systems for auditioning purposes, from studio monitors, hi-fis and headphones, to car stereos, phones, tablets and TVs. ![]() Plug it in the master track of your project as the last segment of your listening chain and quickly turn your studio monitors into classic reference monitors or several consumer devices. This ultimate mixing assistant will help you to get better mixes instantly. ![]() If a slower-paced affair with more transparent options and freedom seems up your alley, then Roguebook might be the game for you. In a similar vein, we’ve seen how the roguelike formula can be applied, so repurposing that in a manner that fits Faeria’s preestablished lore and art should be a cinch.īelieve it or not, Roguebook is just about as successful a product as its esteemed pedigree might imply, but it’s not the be-all and end-all experience one might be looking for from a deck-building roguelike/lite, though that might depend entirely upon how familiar you are with other games of this style. What could possibly go wrong? Well, with a title that literally features the word “rogue,” one might expect a certain style of gameplay and progression to be on display. Roguebook is the brainchild of Richard Garfield, who some might know as the guy who made that sort of popular card game Magic: The Gathering, and Abrakam, the developers of the already successful Faeria (also on the Switch). A deeper story, more variety in levels and characters, and some crucial bug fixes would go a long way in giving Roguebook long-lasting appeal, but it's still well-worth checking out for fans of either roguelikes or deckbuilders - as long as they can stomach a few crashes and don't mind a bit of an easier experience than they may be used to.Resident roguelike/deck-building expert Evan here, ready to report my findings on the latest addition to the Switch library. It's a very good deckbuilding game hiding within an innovative take on roguelike play, and the charm of its characters and the accessibility of its difficulty will go a long way in compelling people to give it a try. It's hard to imagine, however, without some content updates in the near future and a new hero or two, that Roguebook will continue to challenge and captivate players for as long as titles like Slay the Spire have.Įrrors aside, though, Roguebook is still an exceptionally fun experience. Mileage will vary, of course, and the game features a compelling engine that makes exploring card combinations fun. Players can reasonably expect to beat the game in a few dozen hours, which is a decent amount of time but far less than many of the genre's offerings. As far as roguelikes go, Roguebook is pretty easy. Other crashes, like ones at loot collection, came up but with less frequency.īeyond the crashes, the most divisive element of Roguebook will likely be its difficulty and, as a result, its length. ![]() While reloading and not playing the card at that specific time fixed some of the crashes, it did negatively impact strategic play, which is especially egregious in a game where that's fundamentally what players have come to engage with. Some cards played at a specific time crashed the game, and continued to do so on reloads, though Roguebook thankfully saves progress frequently and any crashes generally required completing the most recent action once more. The most noticeable flaws are behind the scenes of the title, which experiences fairly frequent crashes at time of writing. Roguebook is not a perfect game, however. Related: Neoverse Trinity Edition Review: Deckbuilding Depth and Anime Girls In this, the game feels significantly more balanced, with a slew of exciting cards available that feel mechanically distinct from each other and allow for some exciting play patterns. The actual process of building a deck in Roguebook is exciting, with players acquiring cards during their exploits and carefully working out a strategy from what's available. Some equipment, like an Epic item that doubles the damage of the first spell played each turn, outright trivializes most runs, while many of the gems feel largely useless at common rarity - and generic, which is perhaps a greater sin in a genre so full of innovation. ![]() There are more familiar roguelike trappings here, too, like equipment and card modifications, though these feel a little less balanced than the cards themselves. ![]() When we provide you with a product or service, we make a commitment we call the Mustang Promise. As a client of The Mustang Cat Rental Store, you also receive the comprehensive product support associated with our name. Every piece of used equipment has a full maintenance history. Our team puts as much care into our used products as we do our new purchases. Buy From Our El Campo Rental Fleetĭo you have plans to buy a used Cat machine? We sell used equipment at our El Campo location that comes from our rental fleet.
![]() With the increasing expectation of GD 2 as a broad target for CAR T cell therapy and the expected benefit in applying TRUCKs with transgenic IL-18 release, there is a need to manufacture such cellular medicinal products in a safe, validated and reproducible fashion. Initial GD 2-CAR T cell clinical studies targeting neuroblastoma by first to third generation CAR T cells showed moderate or transient anti-tumor responses but failed to produce sustained remissions, emphasizing the need to modulate the T cell response ( 17– 19). GD 2 therefore is a promising target for redirected immunotherapy. GD 2 is highly and consistently expressed in childhood cancer neuroblastoma and can be found on the cell surface of other solid cancer entities including breast cancer ( 8), osteosarcoma ( 9), melanoma ( 10), glioblastoma ( 11), small cell lung cancer ( 12), retinoblastoma ( 13), soft tissue sarcoma ( 14) and Ewing sarcoma ( 15, 16). Physiological expression of GD 2 is restricted to low densities on neurons, skin melanocytes and peripheral pain fibers ( 7). In this study we focused on the manufacturing of TRUCKs targeting disialoganglioside GD 2. CAR T cells engineered with inducible IL-18 release improve T cell effector functions towards superior activity against pancreatic and lung tumors in mice that were refractory to CAR T cells without cytokines ( 5, 6). Innate immune cells are attracted and activated by IL-12 or IL-18 ( 4) to eliminate antigen-low expressing or antigen-negative cancer cells within the tumor ( 2). These factors include cytokines, such as IL (interleukin)-12 and IL-18, but also enzymes and costimulatory ligands augmenting T cell activation. TRUCKs are thereby used as “living factories” to produce and deposit substances with anti-tumor activity in the targeted tissue. TRUCKs are CAR T cells that release a transgenic protein upon CAR engagement of cognate antigen and signaling. A promising strategy to target solid tumors with their phenotypic heterogeneity has led to the fourth generation of CARs also known as TRUCKs or armored CARs ( 2, 3). To enable and improve CAR T cell proliferation, anti-tumor activity, and in vivo persistence, advanced generations of CARs have been developed ( 1). One of the most significant recent developments in cancer therapy is the CAR T cell technology. In conclusion, GMP-compliant manufacturing of TRUCKs is feasible and delivers high quality T cell products. Following antigen-specific CAR activation of TRUCKs, CAR-triggered release IL-18 was induced, and the cytokine was biologically active, as demonstrated in migration assays revealing specific attraction of monocytes and NK cells by supernatants of TRUCKs co-cultured with GD 2-expressing target cells. Manufactured TRUCKs showed a specific cytotoxicity towards GD 2-expressing target cells indicated by lactate dehydrogenase (LDH) release, a decrease of target cell numbers, microscopic detection of cytotoxic clusters and detachment of target cells in real-time impedance measurements (xCELLigence). IL-2, granzyme B, IFN-γ, perforin, TNF-α). CD25, CD69) on both CD4 + and CD8 + T cells and an enhanced release of pro-inflammatory cytokines and cytolytic mediators (e.g. IL-18 TRUCKs were activated in an antigen-specific manner by co-cultivation with GD 2-expressing target cells indicated by an increased expression of activation markers (e.g. The functional capacities of the clinical-scale manufactured TRUCKs were similar to TRUCKs generated in laboratory-scale and were not impeded by cryopreservation. Preclinical characterization demonstrated antigen-specific GD 2-CAR mediated activation after co-cultivation with GD 2-expressing target cells. Transduction efficiencies of 77.7% and 55.1% was obtained, and yields of 4.5 x 10 9 and 3.6 x 10 9 engineered T cells from the two donors, respectively, within 12 days. Starting with 0.84 x 10 8 and 0.91 x 10 8 T cells after enrichment of CD4 + and CD8 + we reached 68.3-fold and 71.4-fold T cell expansion rates, respectively, in two independent runs. We generated IL-18-secreting TRUCKs targeting the tumor antigen GD 2 using the CliniMACS Prodigy ® system using a recently described “all-in-one” lentiviral vector combining constitutive anti-GD 2 CAR expression and inducible IL-18. ![]() ![]() Based on our previous development and validation of automated and closed processing for GMP-compliant manufacturing of CAR T cells, we here present the proof of feasibility for translation of this method to TRUCKs. Thus, approaches using 4 th advanced CAR T cells secreting immunomodulatory cytokines upon CAR signaling, known as TRUCKs (“ T cells redirected for universal cytokine-mediated killing”), are currently under investigation. Chimeric antigen receptor (CAR)-engineered T cells can be highly effective in the treatment of hematological malignancies, but mostly fail in the treatment of solid tumors.
![]() You can read the full details in the link below. You are only allowed to create maps and you can neither save nor share them. It also has a free version, however, it is fairly restricted. There are dozens of themes to chose from and each one looks better than the other. You can change the theme of your mind maps at any given time. You can choose from pre-formatted templates or you can create you own from scratch. It also allows you to directly share your mind maps to your Evernote account. You can share it or print it into PDF of OPML. You can create mind maps, attach photos, links, and notes. Of course, it does everything a mind mapping software is supposed to do. XMind got itself on this list due to its theming capability. Install: Free, premium plans start at $10/month 3. Thankfully, there’s a generous free tier so you can check it out without paying a penny. You can also use it as an individual, but it makes more sense for a team. Overall, I would say Ayola is great for teams that want to break out from mundane project planning and use a more visual approach. You get a visual task manager along with built-in team chat features. If you are in a hurry to jot down ideas, this is the perfect place to start.Īyola also brings productivity features for teams such as a Gnatt timeline view that allows you to create Gnatt chats for easily managing time. There’s also something called speed mind mapping, that allows you to quickly create mind maps by stripping all the extra features and serving you with basic mind mapping features. What’s great here is that you can share these mind maps with your co-workers, allowing you to easily communicate your thoughts and brainstorming process. Then there are normal mind maps that you can create by using nodes and connecting lines. This is great for organizing as it gives you a broad overview of your goals and projects. One of the beloved features of Ayoa is the radial maps feature that allows you to create mind maps in concentric circles. Where is MindNode is for users who are looking for a fluid and simple mind mapping experience, Ayoa is for individual and teams that need all the features. Previously known as iMindMap - Ayoa, is one of the most powerful mind mapping applications on the market right now. Its’ one of the best mind mapping apps for Mac. MindNode is what a great Mac app looks and feels like and I like it. You also get awesome features including the ability to create outlines, a quick entry shortcut for quickly adding ideas, support for stickers and themes, visual tags, notes, and more. The nodes are foldable hence allowing to create a large mind mapping tree without being overwhelmed by the data. Its interface is designed in such a way that you are not losing much functionality even if you are using it on mobile devices. It also has iCloud sync and an app for iPad and iPhone. If you are just starting out, MindNode will help you learn the basics of the mind mapping technique without in your face instructions. It has a beautiful UI and works buttery smooth. If you want an app that is build for Mac and brings a fluid mind-mapping experience, MindNode is the one for you. So check out these apps and start mind-mapping today. Whether you are planning your next trip, writing a book, or creating a presentation for your next project, mind-mapping can help you organize your jumbled mess of a head into something that looks coherent and executable. ![]() “Come on, take a bite.” He brings it closer to your lips and you bite it, revealing the pink sprinkles inside the cupcake. “Cupcake, baby?” Your husband reaches out his hand to grab one and offers you the one with the white frosting on. ![]() “My god… she loves it so much, y/n look.” He calls out for you to show you how big her smile is whenever she sees herself in the phone screen, making you melt even more and you lean in to place a peck on her chubby cheeks. When you come back to the living room you notice the selfie lover in your group of friends immediately took out his phone and took some selfies with your daughter who was still in her daddy’s arms. “Well thank god my husband is not you, I’d have much uglier kids then.” You talk back to him, making everyone laugh even louder at your savage reaction while putting down your babyboy to sleep, covering him with a tiny blanket they bought for the kids. “AW, Look how ugly, she looks just like you y/n!” The tease one states, making everybody laugh, even your husband who tried his best to hide his little giggle that left his lips. You take a look at your husband, he wasn’t smiling like this even on your wedding day, which was his first best day of his life, as well as yours. “Aww… look at you babygirl!” Your husband’s best friends takes a closer look at your daughter who is in her father’s arms, smiling like the sweet little Angel she is whenever someone approaches. “But he’s been crying the whole day, so if he really wakes up because of you that’s it for you.” You continue, lowering your voice as well. “Thank you guys, I love you all.” That’s the first time in a while you’re being serious with them. “You know, that’s a weird way to thank us y/n…” one of them says, holding a blue decorated cupcake. You take a quick look at your baby who was the sweetest copy paste version of your loved one, your husband. “If he’s waking up because of y’all I’m kicking you out of the window.” You joke sarcastically, giving them all a death glare and receive some laughter from them. His smile has never been wider than now because of the huge surprise party they held for the fourth of you. ![]() “Welcome back home!” Confetti is thrown everywhere once you step in, tightening the grip on your baby boy who just fell asleep on your shoulder, eyes widen at your not as organized as you left your house before heading to the hospital and your husband shouts back at the members as an instinct. The moments until you both open the door feels like infinity for the boys because they were waiting for this moment since this morning. “You think or you checked with him?” The older tells him, ready to fight but your footsteps distract them all, catching their attention from whatever balloon they were sticking to the wall or whichever number of cupcake they shove into their mouth and swallowed within a second. “Shh… I think they’re coming!” The youngest yells (if your bias is the youngest then it’s the second youngest). ![]() Requested ✔️ by female reader x optional bias ![]() some of the most popular teas in the world are assam, ceylon, darjeeling, nilgiri, keemun (china) lapsang souchong, zunan, irish breakfast and jasmine to name a few. oolong (a kind of tea that is partly fermented before being dried) is produced just like black tea, except that the leaves are oxidised for less time. the basic process of black tea involves withering, rolling, oxidation and firing. the orthodox method involves a greater manual process and differs for black, green and oolong teas. but tea connoisseurs turn up their nose at ctc tea. ctc stands for 'crush, tear and curl', and it's a mechanised process, after the leaves have been picked, and then it's 'fired' or dehydrated. the harvested crop is processed in two ways: ctc or orthodox. the first flush, usually in early march, yields the best crop. harvesting seasons last generally from march to november. ![]() the crop is usually trimmed and kept at arms length in order to make it easier for plucking. broadly speaking, the first step towards getting good tea lies in the harvesting. tea was first cultivated and grown in assam by robert bruce, an adventurer and trader. the leaves that please the tea plant camellia sinensis is indigenous to india and at one time, grew wild in the foothills of the himalayas. in mainland china, the word used for tea is 'cha', while in russia it is 'chai' as also in india. the first mass marketers of tea were the dutch because fiji was one of the early dutch colonies where tea was grown as a cash crop. in fact, the word tea is derived from fujian 'te' (pronounced tay). the british discovered tea long after the persians, portugese and the dutch. the leaves were then left on a golden platter to dry before being used to make his tea. young virgins wearing gloves and using gold scissors snipped the two freshest leaves on the plant along with a bud. ![]() emperor hui tsun of the 10th century (1100 - 1126), was a connoisseur of tea.stringent imperial plucking methods were used to select the leaves that went to make his 'cup of cheer'. way back when.the story of tea begins in china and goes back almost 5000 years. It helps you start your day: that cup of tea that you cannot do without as you read your morning is the most commonly consumed drink after water, and scientific research has indicated that the regular consumption of tea can have very positive effects on your health and well-being. |
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